Journal:

Article Title: Genes Specific for the Biosynthesis of Clavam Metabolites Antipodal to Clavulanic Acid Are Clustered with the Gene for Clavaminate Synthase 1 in Streptomyces clavuligerus

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Production cultures were grown under conditions similar to those used for seed cultures. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Reference or source Strains Bacillus sp. ATCC 27860 Indicator strain; sensitive to alanylclavam on minimal medium 26 E. coli ER1447 ara-14 F − dam-13 ::Tn 9 (Cm r ) dcm6 fhuA31 galK2 galT22 hisG4 hsdR2 (r k − m k + ) lacY1 leuB6 mcrA mcrB1 mtl-1 rpsL136 (Str r ) supE44 thi-1 tsx-78 xyl-5 J. McCormick, Harvard University, Cambridge, Mass.

Techniques: Plasmid Preparation, Northern Blot, Clone Assay, Isolation, Modification

Journal:

Article Title: Mutants of Streptomyces clavuligerus with Disruptions in Different Genes for Clavulanic Acid Biosynthesis Produce Large Amounts of Holomycin: Possible Cross-Regulation of Two Unrelated Secondary Metabolic Pathways

doi: 10.1128/JB.184.23.6559-6565.2002

Figure Lengend Snippet: Strains used in this work

Article Snippet: To purify holomycin, TSB medium was inoculated to 5% (vol/vol) with a seed culture in the same medium, and the culture was incubated for 48 h under the conditions indicated above. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strains Characteristics or use Origin or reference S. clavuligerus ATCC 27064 Wild type ATCC a ccaR :: aph Cephamycin C, clavulanic acid-nonproducer (CCA − ), disruption in ccaR , kanamycin resistant (Km r ) 25 claR :: aph Clavulanic acid nonproducer (CA − ), disruption in claR 26 ORF6:: apr Disruption in ORF6, apramycin resistant (Apr r ) 7 ORF6:: apr ccaR :: aph CCA − , disruption in ccaR and ORF6, Km r Apr r 7 ceaS :: aph CA − , disruption in ceaS , Km r 27 RLF3 CA − , disruption in bls , Km r 2 car :: aph CA − , disruption in car , Km r 26 cyp :: aph CA − , disruption in cyp , Km r 17 ORF12:: aph CA − , disruption in ORF12, Km r 17 ORF15:: aph CA − , disruption in ORF15, Km r 17 ORF15:: aph (pIJ699) CA − , disruption in ORF15, Km r Thio r 16 ORF15:: aph (pIJ699-ORF15) CA − , disruption in ORF15, complemented, Km r Thio r 16 cyp :: aph (pVK99) CA − , disruption in cyp , Km r Thio r 16 cyp :: aph (pVK99- cyp ) Disruption in cyp , complemented, Km r Thio r 16 Micrococcus luteus ATCC 9341 Sensitive strain in bioassay ATCC Bacillus sp. strain 27860 Sensitive strain in bioassay ATCC Escherichia coli Ess22-31 Sensitive strain in bioassay 26 Klebsiella pneumoniae 29665 Sensitive strain in bioassay ATCC Alcaligenes faecalis Sensitive strain in bioassay Laboratory stock Enterobacter cloacae P 99 Wide-spectrum cephalosporinase producer Laboratory stock Open in a separate window a ATCC, American Type Culture Collection.

Techniques: